Find the concentration of sucrose in a potato cell - Plan :: GCSE Biology A2 A-Level Coursework

Find the concentration of sucrose in a potato cell - Plan Plan For Osmosis/Plasmolysis Experiment AIM In this experiment, I am trying to find the concentration of sucrose in a potato cell. Also I can observe when a cell is totally plasmolyised. PLAN I plan to complete this experiment within an hour. Firstly, I will cut 15 potato chips, 3 each for 5 different solutions, each weighing 1.2grams, cut with a 4mm corer to approx (full word) 4cm. I will measure weight, as length is less accurate. The weight of the chip may vary as much as 0.02 g each way, which will make it a lot easier to do, but may provide me with less accurate results. I will use 5 different molar solutions of 20 cm3 each, shown to the right. 0M (water), 0.5M, 1M, 1.5M and 2M. I will create 0.5 molar solutions by mixing 10cm3 of water and 10cm3 of 1 molar solution. I will use 3 of each solution to ensure that my results are correct, and to prevent any anomalies disturbing my result graphs. One of each solution will be dropped into the solution at 10-second intervals. They will be taken out 30 minutes after precisely, to ensure a reliable result. They will then be dried on a paper towel, and quickly weighed, and recorded in a pre-written table. RELIABILITY/ FAIR TEST I must control many variables in this test. I will control the time that they are left in the solution, as this will greatly affect results if they are left in for different amounts of time. Also I must control how much I dry the chips before weighing them, because if I leave some excess water on one of them, it will be an unfair test, as ill be weighing the water as well as the chip. Another point is simple, but just to make sure that the scales I am weighing the chip on are at 0 before weighing the chip, as it may add some mass to the chip. I will use 5 different solutions, starting at 0 molar and going up in 0.5 molar concentrations. I have chosen this because they are equidistant, allowing for easy graph drawing, and also because from the preliminary experiment, I know that this will give me results that can show me the concentration of sucrose in the cells sap, and also go on to show me the concentration that the cell will plasmolyise at. I will also control the variety of potato- I will only use 1 brand, as